Microbiome Analysis Of Human Normal Specific Flora From Skin Of Laborers And Academic Professionals Of Lahore For Forensic Application
By: Talha Umair (2014-VA-941)
| Dr. Wasim Shehzad.
Contributor(s): Dr. Saadat Ali | Dr. Muhammad Yasir Zahoor.
Material type: 
BookPublisher: 2016Description: 65p.Subject(s): Forensic SciencesDDC classification: 2639-T Dissertation note: Human microbiota or normal flora is the aggregate of microorganisms that resides on the
surface of skin, oral mucosa, conjunctiva and GIT. Human skin has a complex variety of
microbial system and varieties of microbes mean that they are potential source of forensic
identification because human microbiome varies individual to individual due to differences
in hygiene, professions and region to region because of some environmental factors and
microbial flora can shed more frequently upon touching any kind of surfaces and microbes
are left for long time at any surface so can be identified easily.
Human microbiota varies individual to individual so it may become potential source for
forensic identification of individuals through specific microbiome analysis.
Fourty Samples were obtained by swabbing from the palm surfaces of hands and soles of feet
of individuals of different professional groups in order to recover bacterial communities.
Bacterial culturing and Bacterial DNA extraction followed by the implementation of 16S
rRNA amplification by polymerase chain reaction (PCR) and sequencing of the PCR
product, allowed an even more comprehensive broad range investigation of bacterial
communities. Bioinformatics analysis was done to compare microbial communities.
This research elaborated the significance of skin microbial communities in identifying
individuals and can be a major contribution in forensic science to find and identify
individuals when there is less major evidence, i.e. human DNA and body fluids.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 2639-T (Browse shelf) | Available | 2639-T |
Human microbiota or normal flora is the aggregate of microorganisms that resides on the
surface of skin, oral mucosa, conjunctiva and GIT. Human skin has a complex variety of
microbial system and varieties of microbes mean that they are potential source of forensic
identification because human microbiome varies individual to individual due to differences
in hygiene, professions and region to region because of some environmental factors and
microbial flora can shed more frequently upon touching any kind of surfaces and microbes
are left for long time at any surface so can be identified easily.
Human microbiota varies individual to individual so it may become potential source for
forensic identification of individuals through specific microbiome analysis.
Fourty Samples were obtained by swabbing from the palm surfaces of hands and soles of feet
of individuals of different professional groups in order to recover bacterial communities.
Bacterial culturing and Bacterial DNA extraction followed by the implementation of 16S
rRNA amplification by polymerase chain reaction (PCR) and sequencing of the PCR
product, allowed an even more comprehensive broad range investigation of bacterial
communities. Bioinformatics analysis was done to compare microbial communities.
This research elaborated the significance of skin microbial communities in identifying
individuals and can be a major contribution in forensic science to find and identify
individuals when there is less major evidence, i.e. human DNA and body fluids.
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